Concentration and Purification of Viruses with Special Reference to Reoviruses.
نویسنده
چکیده
INTRODUCTION.................................................................................. 391 METHODS...................................................................................... 391 Ultracentrifugation ............................................. 391 Chromatography.............................................................................. 392 Electrophoresis ............................................. 393 Partition of Liquid Phases............................................. 393 APPLICATIONS TO REOVIRUSES............................................ 393 LITERATURE CITED............................................ 395
منابع مشابه
Human Reoviruses Serotype 3 Effectively Target Huh-7 Cells
Abstract: Background and Aims: Huh-7 is a cell line that was derived from a liver tumor of a Japanese man. Hepatocellular carcinoma (HCC) is considered as a primary liver cancer. Highly resistant tumor to treatment which causes the death of many patients annually. Thus, targeting the cancer cells by using a new method could be effective in...
متن کاملOne-year survey of enteroviruses, adenoviruses, and reoviruses isolated from effluent at an activated-sludge purification plant.
Samples of raw sewage, primary effluent, and secondary effluent from a large activated-sludge purification plant near Melbourne (Victoria, Australia) were collected every second week for 1 year. Viruses were detected in all secondary effluent samples and in six of seven samples obtained after final chlorination. Adenoviruses (85% reduction) and reoviruses (28% reduction) were removed less effic...
متن کاملMechanisms of viral pathogenesis. Distinct forms of reoviruses and their roles during replication in cells and host.
Recent years have seen a resurgent interest in mechanisms by which viruses cause disease, or viral pathogenesis. Studies of viral pathogenesis often address the capacity ofa virus to cause injuries to cells and tissues; yet, injuries are secondary consequences ofmany fundamental interactions that occur between virus and single cells, as well as between virus and host organism, during infection....
متن کاملOptimization of S-layer protein purification from Deinococcus radiodurans strain R1
S-layer proteins of Deinococcus radiodurans are the best self-assemble systems among other proteins that have an essential role in the fabrication of nanowires. Therefore, the purification of these proteins is necessary. The purpose of this research was to optimize the purification of s-layer protein from D. radiodurans with the response surface method. The three factors of SDS concentration, i...
متن کاملDetection and identification of mammalian reoviruses in surface water by combined cell culture and reverse transcription-PCR.
Reoviruses are a common class of enteric viruses capable of infecting a broad range of mammalian species, typically with low pathogenicity. Previous studies have shown that reoviruses are common in raw water sources and are often found along with other animal viruses. This suggests that in addition to the commonly monitored enteroviruses, reoviruses might serve as an informative target for moni...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bacteriological reviews
دوره 28 شماره
صفحات -
تاریخ انتشار 1964